Respuesta :
The study described here demonstrated that S. cerevisiae could produce more FFA by controlling the acyl-CoA metabolism by deleting acyl-CoA synthetase and expressing thioesters, indicating tremendous potential for exploitation in the platform of microbial fatty acid-derived biofuels.
Fatty acid:
- Recent years have seen a lot of interest in the production of biofuels made from microbial fatty acids due to its potential to replace fuels made from petroleum. The flux toward the direct precursor fatty acids needs to be improved to approach high yields in order to be cost-competitive with existing petroleum fuel. In this study, the metabolism of free fatty acids in Saccharomyces cerevisiae was altered (FFA). First, the haploid S. cerevisiae double deletion strain faa1faa4 was created with the deletion of the genes for the two acyl-CoA synthetases FAA1 and FAA4. Then, the strain faa1faa4 expressed the truncated form of acyl-CoA thioesters ACOT5 (Acot5s), which codes for Mus musculus peroxisomal acyl-CoA thioesters 5.
- In comparison to the wild-type strain and the twofold deletion strain faa1faa4, the resulting strain faa1faa4 [Acot5s] collected more extracellular FFA with a higher unsaturated fatty acid (UFA) ratio. During the stationary phase, the strain faa1faa4 [Acot5s] produced 6.43 times as many extracellular total fatty acids (TFA) than the wild-type strain. While no UFA was found in the wild-type strain, UFA made up 42% of TFA in the strain faa1 faa4 [Acot5s]. Additionally, the expression of Acot5s in faa1-faa4 restored the growth, suggesting that FFA may not be the cause of the strain's growth suppression. The de-repression of genes involved in fatty acid synthesis caused a rise in extracellular fatty acids, according to the results of RT-PCR.
- The study described here demonstrated that S. cerevisiae could produce more FFA by controlling the acyl-CoA metabolism by deleting acyl-CoA synthetase and expressing thioesters, indicating tremendous potential for exploitation in the platform of microbial fatty acid-derived biofuels. Recent years have seen a lot of interest in the production of biofuels made from microbial fatty acids due to its potential to replace fuels made from petroleum.
- The flux toward the direct precursor fatty acids needs to be improved to approach high yields in order to be cost-competitive with existing petroleum fuel. In this study, the metabolism of free fatty acids in Saccharomyces cerevisiae was altered (FFA). First, the haploid S. cerevisiae double deletion strain faa1faa4 was created with the deletion of the genes for the two acyl-CoA synthetases FAA1 and FAA4. Then, the strain faa1faa4 expressed the truncated form of acyl-CoA thioesterase ACOT5 (Acot5s), which codes for Mus musculus peroxisomal acyl-CoA thioesterase 5.
- In comparison to the wild-type strain and the twofold deletion strain faa1faa4, the resulting strain faa1faa4 [Acot5s] collected more extracellular FFA with a higher unsaturated fatty acid (UFA) ratio. During the stationary phase, the strain faa1faa4 [Acot5s] produced 6.43 times as many extracellular total fatty acids (TFA) than the wild-type strain. While no UFA was found in the wild-type strain, UFA made up 42% of TFA in the strain faa1 faa4 [Acot5s]. Additionally, the expression of Acot5s in faa1-faa4 restored the growth, suggesting that FFA may not be the cause of the strain's growth suppression. The de-repression of genes involved in fatty acid synthesis caused a rise in extracellular fatty acids, according to the results of RT-PCR.
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