The phase of mitosis in which the centromeres split and sister chromatids are pulled to opposite poles of the cell is Anaphase II
The stage of mitosis known as anaphase occurs after the metaphase process, during which replicated chromosomes are split and the newly-copied chromosomes are transferred to opposing poles of the cell. To aid in chromosome segregation and the re-formation of the nucleus, chromosomes also reach their overall maximum condensation during late anaphase.
The ubiquinylation of securin, an inhibitory chaperone, by the anaphase-promoting complex signals the beginning of anaphase. A protein called securin prevents the activity of a protease called separase. Cohesin, a protein that holds sister chromatids together, is broken down as a result of the breakdown of securin, which releases separase.
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