To better understand and treat caused diseases, a pure bacterial culture is still necessary for the research of its virulence, susceptibility to antibiotics, and genomic sequence.
Incubation time, nutrients, environment, and temperature were empirically changed in the early culture conditions before culture was increasingly replaced by molecular techniques. Microbiologists that specialize in intracellular bacteria were responsible for the resurgence of culture in clinical microbiology. An expansion of the archaea's repertory was made possible by the introduction of a new, adaptable media. However, among common bacterial diseases, the creation of axenic medium for the culture of Treponema pallidum or Mycobacterium leprae continues to be a significant obstacle that cultivators will be able to overcome with time and creativity.
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