The directionality of the polynucleotide allow the base pairing in the chain that is replicated and/or transcribed.
A polynucleotide chain possesses directionality, or two ends that differ from one another, as a result of the structure of nucleotides. The 5' phosphate group of the chain's initial nucleotide protrudes at the 5' end, or commencement, of the chain. The 3' hydroxyl of the final nucleotide added to the chain is exposed at the opposite end, often known as the 3' end.
Typically, DNA sequences are written from 5' to 3'. Base pairing, which is necessary for both the replication and transcription of the genetic material, is made possible by the DNA double helix's two linked strands running in opposite directions. New copies of DNA and RNA nucleotide chains are created from the originals during replication.
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