4-hydroxynitrobenzene will elute last from the silica filled column.
- A scientific method for dissolving a mixture into its constituent parts is chromatography.
- the chromatographic procedure of removing an adsorbate from a solid adsorbing media called elute using a solvent. separating an antibody from the antigen it is bound to.
- In reverse phase liquid chromatography, stationary phase is non-polar in nature and becomes more non-polar as the number of carbon chains grows.
- The order of elution from a column usually follows the series: alkyl halides < saturated hydrocarbons < unsaturated hydrocarbons < ethers < esters < ketones < amines < alcohols < phenols < acidsare typically eluted from columns in the following order. Salts and polymeric substances frequently do not elute. Solvents: For adsorbent sites, the solvent will compete with the sample.
- The more polar the solute, the more readily it will dissolve in the stationary phase, remain in the column, and finally elute last. Therefore, in normal phase chromatography, the non-polar component will elute earlier than the polar component between the polar and non-polar components.
4-hydroxynitrobenzene is more polar than 2-hydroxynitrobenzene therefore it will elute last.
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