Respuesta :
The following errors in the PCR reaction could lead to the result obtained:
He performed step two at a temperature that was too low.
Repeated sequences were present in the designed primers.
He contaminated the DNA sample used as a template.
Polymerase Chain Reaction (PCR) is a molecular biology technique used for the amplification of DNA fragments with the help of Taq polymerase at a high temperature.
When the temperature for step 2 of PCR, annealing is too low, the primers do not get attached to the template DNA.
When repetitive sequences are present in the primers, dimers may form.
Contamination of the template DNA will lead to no PCR and thus little to no results.
The complete question is:
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
After electrophoresing his PCR reaction, the student sees the desired PCR product on the gel as well as several smaller bands. What error(s) might he have made? (Select all that apply.)
Learn more about PCR here:
https://brainly.com/question/12001606
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