Molecular exclusion chromatography can be used to measure the molecular mass of a protein as it separates the proteins on the basis of molecular mass.
Exclusion chromatography refers to those chromatographic techniques that divide sample components based on their molecular weight. Several of these methods are now under development, and it is practical to categorize them into two groups: sieving separations and gel permeation.
The chromatographic technique known as size-exclusion chromatography (SEC), also referred to as molecular sieve chromatography, separates molecules in solution based on their size and, in some situations, molecular weight. Proteins and commercial polymers are typical examples of big molecules or macromolecular complexes to which it is typically applied.
Thus, molecular exclusion chromatography can be used as it separates the proteins on the basis of molecular mass.
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