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an autoradiogram was generated by the dideoxy sequencing method. reading from bottom to top gives you the sequence of the synthesized strand in the 5 to 3 direction. it is 'tgaacggt'. what would the sequence of template strand be from the 5 to the 3 end?

Respuesta :

The template strand is complementary and runs in the opposite direction because the synth strand is 5' - TGAACGGT - 3'. ACTTGCCA - 3' - 5'

In the fundamental dideoxy sequencing reaction, an oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA polymerase. Four deoxyribonucleoside triphosphates (dNTPs), one of which is 35S-labeled, are present while this is being done. Additionally, one of the four ddNTPs, which block elongation when incorporated into the lengthening DNA strand, is present in the reaction. Following the sequencing operations, the results are electrophoresed on a high-resolution denaturing polyacrylamide gel, and the DNA sequence is then visible via autoradiography.

Learn more about dideoxy sequencing at

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