The difference between pcr and sanger sequencing with regard to the materials needed to perform these reactions is that dideoxynucleotides are needed in Sanger sequencing unlike PCR.
Dideoxynucleotides are needed for sanger sequencing, while it is not requires to perform PCR. Two primers from opposing strands are necessary for PCR amplification in order to specify the region of the sequence amplified in both the forward and reverse directions. In contrast to PCR, Sanger sequencing uses just one primer in the process. The fundamental difference between the two is that while PCR is used to duplicate the entire DNA sequence, Sanger sequencing is used to generate every possible length of DNA.
The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA. In this method, target genetic regions are amplified using polymerase chain reaction (PCR), and the amplified products are then sequenced.
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