You are studying a drug that blocks translation in bacteria. You want to know more about its mechanism of action. You treat bacteria with the drug and isolate mrnas with their associated proteins from the treated bacteria. In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mrnas, but not the large subunits. In which stage does this drug arrest translation?.

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15-11-2022
Biology

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You are studying a drug that blocks translation in bacteria. You want to know more about its mechanism of action. You treat bacteria with the drug and isolate mrnas with their associated proteins from the treated bacteria. In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mrnas, but not the large subunits. In which stage does this drug arrest translation?.

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qwmoon qwmoon · Answer 1 · 2022-11-19T13:23:59+01:00

In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mRNAs, but not the large subunits. This drug arrest translation in the termination stage.

The natural compound Blasticidin S, which acts on both prokaryotic and eukaryotic cells and inhibits both peptidyl-tRNA hydrolysis and to a lesser extent peptide bond formation, is the only known small molecule inhibitor that impacts termination.

However, this medication binds to the ribosomal 50S subunit at a place shared with several other antibiotics and functions primarily as a PTC inhibitor that has been demonstrated to deform the CCA end of the P-site tRNA8. Therefore, interference is its way of action.

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