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The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.
Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.
Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.
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This is often done using a denaturing polyacrylamide urea gel, with four reactions each run in one of the four lanes (lanes A, T, G, C). The DNA bands are then visualized by autoradiography or UV light and the DNA sequence can be read directly from the X-ray film or gel image.
In automated Sanger sequencing, a computer sequentially reads each band on a capillary gel and uses fluorescence to determine the identity of each terminal d dNTP. In essence, a laser excites the fluorescent labels in each band and a computer detects the resulting emitted light.
Sanger sequencing produces extension products of variable length that terminate in dideoxy nucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer.
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