In order to make transgenic mice with eyes on their legs the coding sequence of pax6 DNA should include Mouse promoter and Leg Enhancer in DNA.
In the question, it is stated that a pax6 gene is to be ectopically expressed in mouse legs in order to make transgenic mice with eyes on their legs. In order to do so, it is important to include a Mouse promoter and Leg Enhancer in the DNA sequences and fuse them with the pax6 coding sequence.
The Pax6 gene is generally defined as the gene that is responsible for the physical development or defines the tissues and the organs during the embryonic development of a specie.
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