a student has everything he needs to make cdna of a gene of interest. he starts with an sample of mrna from the cells containing the gene, and proceeds with the experiment. at the end of the procedure, he finds that the molecule he has synthesized contains uracil. what was his mistake?

Respuesta :

The student has made a mistake in the synthesis of cDNA. The cDNA molecule should contain thymine, not uracil.

Uracil is found in RNA, not DNA. DNA contains thymine. The cdna molecule will not be able to bind to the dna template. This will result in a lower yield of cDna and potentially lower quality cDNA. If the mRNA sample is not pure, then other nucleic acids present can be reverse transcribed into cDNA, resulting in a final molecule that contains uracil.

Another possibility is that the student did not properly prime the reaction, meaning that they did not add the necessary nucleotides and enzymes for the reaction to occur. without a proper primer, uracil can be incorporated into the cDNA molecule.

Learn more about cDNA at : https://brainly.com/question/14006268

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