Student 2 employed the correct scientific reasoning which states that a TLC plate might be used to run an unknown drug, and the spots could then be compared to another TLC plate using known compounds.
Chromatography is a biophysical method used for the separation, identification, and purification of different compounds present in mixtures. The main separating components are stationary and mobile phases. TLC stands for thin-layer chromatography. It uses a TLC plate to separate compounds. A TLC plate is made by applying a thin layer of adsorbent on a sheet. It can be carried out on an analytical scale to track the development of a reaction or on a preparative scale to purify minute quantities of the compound. Because of its simplicity, comparatively low cost, great sensitivity, and rapid separation, TLC is an extensively used analytical method. Similar to all chromatography, TLC works on the premise that a compound will have varying affinities for the mobile and stationary phases, which will influence how quickly it migrates. TLC aims to produce well-defined, well-separated spots.
The question is incomplete. The complete question is as follows;
Three students are asked to discuss how thin-layer chromatography could be used to aid in the identification of a compound. which student employs correct scientific reasoning?
Student 1: An unknown compound could be used as the solvent in the TLC plate to determine its identity.
Student 2: An unknown compound could be run on a TLC plate and then the spots could be compared to separate TLC plates of known compounds.
Student 3: An unknown compound could be run on the same TLC plate as a known compound to see if they are the same chemical.
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