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As part of your research, you examine mRNA expression for the parents, their two unaffected children, and their affected child with a Northern blot using a full-length cDNA probe.

Your results, shown here, are as follows:

Both parents have low, but detectable, mRNA expression.

The unaffected children have either normal or low mRNA expression.

The affected child has no detectable mRNA expression.

What possible molecular defect could explain these results?

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The recessive allele could be a mutation in the transcribed region that makes the mRNA unstable and causes it to degrade.

What possible molecular defect could explain these results?

The recessive allele could be a mutation in the promoter region that prevents RNA polymerase II from binding.The recessive allele could be a mutation in regulatory DNA that prevents chromatin from being remodeled from a closed to an open state.

What is the role of mRNA and tRNA in translation?

Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps decode a messenger RNA (mRNA) sequence into a protein. tRNAs function at specific sites in the ribosome during translation, which is a process that synthesizes a protein from an mRNA molecule.

Learn more about nucleic acid here:

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