blank: a sample created without the absorbing agent utilizing only the solvents and any other compounds present in the sample solutions.
A technique called spectrophotometry uses light intensity measurements as a ray of light travels through a sample solution to determine the amount that a chemical compound absorbs light.
The amount of light absorption by a solution is measured by its absorbance (A), often referred to as optical density (OD).The amount of light that may flow through a solution is called its transmittance.
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