The following which is a limitation of the autoclave is D. It cannot be used with non-heat resistant materials.
An autoclave is a steam machine used to sterilize laboratory equipment. This steam machine, of course, uses steam to carry out sterilization so that viruses, bacteria, fungi, and other organisms can die.
Some of the other functions of the autoclave are:
Learn more about viruses here :
https://brainly.com/question/2495832
#SPJ4