Respuesta :
It is important not to dilute the initial sample before it has been loaded on chromatography column, it is crucial to avoid dilution in order to reduce the matrix effect and prevent detector saturation.
Additionally, it is done to guarantee that saturation stays within the calibration curve's acceptable range. The solvent level must be lower than the TLC's starting line in order to prevent the spots from dissolving. It is imperative that you just use a few drops of solvent to load the sample in this situation if you choose to load the column wet. If you utilise too much solvent, the loading solvent will obstruct the elution and, as a result, the mixture's separation. Because veins and bubbles will form and lead to poor separation, the solvent drop must not extend below the top of the adsorbent.
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