) a mutation within the gal80 gene that blocks the ability of gal80 protein to interact with gal3p. (d) a deletion of one of the four uasg elements upstream from the gal1 gene. (e) a point mutation in the gal1 core promoter that alters the sequence of the tata box

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06-12-2022
Biology

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) a mutation within the gal80 gene that blocks the ability of gal80 protein to interact with gal3p. (d) a deletion of one of the four uasg elements upstream from the gal1 gene. (e) a point mutation in the gal1 core promoter that alters the sequence of the tata box

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contexto1220 contexto1220 · Answer 1 · 2022-12-08T19:27:39+01:00

The gal80 gene that blocks the ability of gal80 protein to interact with gal3p by the activation of gal80 gene.

In Saccharomyces cerevisiae, galactose turns on the allosteric sign transducer Gal3 protein (Gal3p), which in flip inactivates the repressor Gal80p, thereby activating the Gal4p-established transcriptional activation of GAL genetic switch.

If the GAL80 gene product can not engage with Gal3p, there may be no interplay with the Gal4p/Gal80p complicated and consequently no GAL1 transcription. In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, stopping GAL gene expression. Galactose triggers an affiliation among Gal3 and Gal80, relieving Gal80 inhibition of Gal4.