The DNA is moved by the RNA polymerase in the 5' to 3' direction. The RNA molecule initially develops its 3' end. At a "start codon," transcription always begins.
A promoter sequence must first be bound by the RNA polymerase. RNA polymerase produces a corresponding RNA transcript to the DNA template strand in the 5' to 3' orientation. The DNA double helix is opened as it advances down the template strand in the 3' to 5' direction. These neighboring genes' transcription factor jigsaw puzzles are mirror images of one another. Additionally, the RNA polymerase is instructed by the direction they point in which direction to begin reading.
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