Because smaller proteins can travel through the gel matrix more quickly, the smaller protein (myosin light chain) will migrate the fastest.
Two molecules with a molecular weight of 200,000 make up the myosin molecule, together with a number of smaller polypeptides with molecular weights between 15,000 and 25,000 daltons. The quality of the protein (a damaged result will produce a smear and diminish the intensity), the specificity of the antibody, and the performance quality (the use of blocking solution and several washes will reduce background noise) all affect the intensity of the protein band. Actin and myosin filaments are the two major elements of vertebrate striated muscle sarcomeres. The diameter of the myosin filament is around 300, and its axial repetition measures 1.6 m.
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