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describe the construction of a recombinant plasmid containing the gene for luciferase and the insertion of the plasmid into a bacterial cell by placing the steps in order.a. Perform a restriction digestion of the luciferase gene and the plasmid. b. Transform bacteria with the recombinant plasmid using electroporation. c. Ligate together the luciferase gene and the plasmid to generate a recombinant plasmid. d. Use PCR to amplify the gene for luciferase. Plate the bacterial cells, and screen for positive transformants.1. First step 2. Last step

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The steps for constructing a recombinant plasmid containing the gene for luciferase and inserting the plasmid into the bacterial cell are:

  • Use PCR to amplify the gene for luciferase.
  • Perform restriction digestion of the luciferase gene and the plasmid.
  • Ligate together the luciferase gene and the plasmid to generate a recombinant plasmid.
  • Transform bacteria with the recombinant plasmid using electroporation.
  • Plate the bacterial cells, and screen for positive transformants.

Therefore, the correct order is D, A, C, B, and E

Plasmids are used by researchers to insert DNA from other sources into plasmids to form recombinant DNA molecules. Bacterial cells that have been transformed with recombinant plasmids will become recombinant bacteria.

Transformation is the process of introducing foreign DNA into a cell. Bacterial transformation with plasmids is important not only for studying bacteria but also because bacteria are used as a means of plasmid storage and reproduction. Therefore, nearly all plasmids (even those designed to be expressed in mammalian cells) contain both the bacterial origin of replication and antibiotic resistance genes for use as selectable markers in bacteria.

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