Plasmid vector that has an E.coli promoter at the top of the restriction site known as expression vector.
Plasmids are extrachromosomal DNA that is usually circular in shape, and these plasmids are found in bacterial cells. Plasmids as vectors are often used to reproduce target genes that express certain phenotypes such as antibiotic resistance, receptor genes, or other proteins. Plasmids replicate independently of the organism's genome and can be engineered to become vector clones.
In order to create a recombinant plasmid that contains the desired foreign DNA properties, splicing of the foreign DNA with the existing plasmid is carried out. Plasmid cutting to be used as a vector with the same restriction enzyme, namely E. Coli. This E.coli motor plasmid vector is called an expression vector. Expression vectors are a type of cloning vector, which contain expression signals that match gene expression maximally. Expression vectors consist of regulatory sequences such as enhancers and promoter regions, which lead to efficient gene expression.
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