what are two basic ways to measure the enzyme activity of any enzyme? b. based on your answer for part a, how did you determine that the enzyme amylase was functioning? c. what is the purpose of dns in monitoring the activity of the enzyme? be specific. d. what is the original color of dna, what color is an indication that the enzyme activity is taking place?

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08-12-2022
Biology

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what are two basic ways to measure the enzyme activity of any enzyme? b. based on your answer for part a, how did you determine that the enzyme amylase was functioning? c. what is the purpose of dns in monitoring the activity of the enzyme? be specific. d. what is the original color of dna, what color is an indication that the enzyme activity is taking place?

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Qwclear Qwclear · Answer 1 · 2022-12-15T10:04:49+01:00

The methods which are employed for measuring the enzymatic activities are known as spectrophotometry, fluorescence, and radiolabeling.

To test the functioning of amylase, the amylase test is performed by clinicians. If the amylase test detects high level of amylase in blood or urine, then it indicates pancreas disorder. In amylase solution, add 2-3 drops of 10% iodine solution directly onto the edge of colonies and Wait 10- 15 minutes and record the results. If the medium turns dark then it shows positive result. The dinitro salicylate or DNS method determines the reducing sugars liberated by the action of hydrolase enzymes on carbohydrates, under standard pH and temperatures. The enzyme activity can be detected by the presence of dark violet color which appears because of oxidation reaction.

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