Three students are asked to discuss the sources of error that might have affected the outcome of the lab. Select the student that employs correct scientific reasoning.
Student 1: If the spot was placed below the solvent level, this would cause the sample to be dissolved into the solvent pool before traveling up the plate.
Student 2: If the solvent used has the opposite polarity of the stationary phase this will cause unequal movement of the sample.
Student 3: If the developing chamber was closed too quickly than the sample wouldn't be able to travel on the TLC plate.

Question

08-12-2022
Chemistry

contestada

Three students are asked to discuss the sources of error that might have affected the outcome of the lab. Select the student that employs correct scientific reasoning.
Student 1: If the spot was placed below the solvent level, this would cause the sample to be dissolved into the solvent pool before traveling up the plate.
Student 2: If the solvent used has the opposite polarity of the stationary phase this will cause unequal movement of the sample.
Student 3: If the developing chamber was closed too quickly than the sample wouldn't be able to travel on the TLC plate.

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AnnuG AnnuG · Answer 1 · 2022-12-14T16:21:00+01:00

Student 1 is correct. He discovered the mistake as;

If the spot was placed below the solvent level, this would cause the sample to be dissolved into the solvent pool before traveling up the plate.

TLC should be positioned so that just the lowest edge of the plate touches the solvent.

Differential affinities (strength of adhesion) of the analyte components towards the stationary and mobile phases result in differential separation of the components. Affinity, in turn, is determined by two molecular properties: adsorption and solubility.

Adsorption is the property of how well a component of a mixture clings to the stationary phase, whereas solubility is the quality of how well a component of a mixture dissolves in the mobile phase.

The greater the adsorption to the stationary phase, the slower the molecule will move across the column.

The more the molecule adsorbs to the stationary phase, the slower it moves across the column.

The greater the molecule's solubility in the mobile phase, the faster it will travel across the column.

Thus, Student 1 is correct. He detected the error by placing the area below the solvent level, which caused the sample to disintegrate into the solvent pool before migrating up the plate.

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