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Operon is a stretch of DNA consisting of an operator, a promoter, and genes for a related set of proteins, usually making up an entire metabolic pathway.

In genetics, an operon is a functional unit of DNA that contains a cluster of genes controlled by a single promoter. The genes are transcribed into a single strand of mRNA and either translated together in the cytoplasm or spliced to produce mono-cistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product. As a result, the operon's genes are either expressed together or not expressed at all. An operon is defined by the co-transcription of several genes.

Operons can also be found in viruses like bacteriophages. T7 phages, for example, have two operons. The first operon encodes a variety of products, including a unique T7 RNA polymerase that can bind to and transcribe the second operon. The second operon contains a lysis gene, which causes the host cell to burst.

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