If two molecules are doubled four times, 32 DNA molecules are created. After 'n' rounds of the polymerase chain reaction, it is possible to produce 2n DNA molecules from a single DNA template molecule.
What is DNA molecules?
Each cycle sees a doubling of the amount of double-stranded DNA, resulting in 2n copies of the DNA after n cycles. The number of DNA molecules (per gram) in your DNA sample can be calculated by multiplying the DNA amount in ng by Avogadro's number, for instance, after 4 cycles you have 16 copies, after 20 cycles you have roughly one million copies, etc (6.022 x 10e23). Each PCR reaction employs two primers, which are created to surround the target region.
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