Investigating Peptidoglycan Recycling Pathways in Tannerella forsythia with N-Acetylmuramic Acid Bioorthogonal Probes

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09-12-2022
Biology

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Investigating Peptidoglycan Recycling Pathways in Tannerella forsythia with N-Acetylmuramic Acid Bioorthogonal Probes

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charithran8975 charithran8975 · Answer 1 · 2022-12-17T16:59:42+01:00

Because T. forsythia cannot make the N-acetylmuramic acid (NAM) sugar needed for peptidoglycan biosynthesis on its own, it must scavenge.

Using N-Acetylmuramic Acid Bioorthogonal Probes to Examine Tannerella forsythia Peptidoglycan Recycling Pathways. The NAM recycling and peptidoglycan scavenging routes of T. forsythia and other cohabiting organisms will be investigated further using this strain. Here, we investigate the NAM uptake recycling ability of T. forsythia using a bioorthogonal alteration to its peptidoglycan that enables click-chemistry. With a bioorthogonal alteration of T. forsythia's peptidoglycan, we are able to insert TreAz into cell surface glycolipids and then bioorthogonal ligate it with alkyne-modified probes to illuminate K.; this allows us to examine the recycling capability of T. forsythia for NAM absorption. Investigating Peptidoglycan Recycling Pathways in Tannerella forsythia, Sharma, A., Grimes, C.L.

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