What steps could help refine the researchers' experiment to modify only the two target genes? Select all that apply.
1. Engineer a version of Cas9 that demonstrates higher fidelity to the target sequence.
2. Inject the plasmids into younger mouse embryos so more cells are modified.
3. Insert more Cas9 and sgRNA during the initial introduction of the components.
4. Make stable transgenic lines so the cutting by Cas9 is not transient.
5. Screen the rest of the mouse genome to be sure the 20-bp targets do not appear elsewhere in the genome.

Question

09-12-2022
Biology

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What steps could help refine the researchers' experiment to modify only the two target genes? Select all that apply.
1. Engineer a version of Cas9 that demonstrates higher fidelity to the target sequence.
2. Inject the plasmids into younger mouse embryos so more cells are modified.
3. Insert more Cas9 and sgRNA during the initial introduction of the components.
4. Make stable transgenic lines so the cutting by Cas9 is not transient.
5. Screen the rest of the mouse genome to be sure the 20-bp targets do not appear elsewhere in the genome.

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Qwpanda Qwpanda · Answer 1 · 2022-12-17T17:59:58+01:00

The component of CRISPR/Cas-9 genome altering can be for the most part separated into three stages: acknowledgment, cleavage, and fix. The planned sgRNA coordinates Cas-9 and perceives the objective succession in the quality of interest through its 5ʹcrRNA correlative base pair part.

Here we survey three basic innovations — grouped consistently interspaced short palindromic rehashes (CRISPR)- CRISPR-related protein 9 (Cas9), record activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). At the point when the objective DNA is found, Cas9 - one of the catalysts delivered by the CRISPR framework - ties to the DNA and cuts it, stopping the designated quality. Utilizing adjusted adaptations of Cas9, specialists can actuate quality articulation as opposed to cutting the DNA. These procedures permit specialists to concentrate on quality capability.

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