blocks TRAP pathway:peptide 2\
blocks agr pathway:peptide 1, peptide 3
blocks both pathways:n/a
Exercising mutants that are now hindered for one of the pathways is a straightforward system for figuring out which pathway every peptide follows up on. Peptides 1 and 3 both inhibit poison stashing in the TRAP- mutant, which has a working agr pathway. thus, you know that peptides 1 and 3 block the agr pathway.
By relative explanation, peptide 2 inhibits poison stashing in the agr- mutant, which has a performing TRAP pathway. thus, you know that peptide 2 blocks the TRAP pathway.
Since you have distinguished important in vitro impediments of TRAP and agr maturity detecting pathways, you choose to test their handiness in live towel exercising and in vivo mouse model.
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