For each of the following categories of compounds, indicate the type of liquid chromatography that could be used to separate analytes in that category. (Drag the appropriate chromatography label to the blank next to the category. Only one label applies to each category, but a label may be used more than once).
Seperation Mode Analyte Category
Fm>2000,soluble in octane,size10 nm
Fm>2000, soluble in butanol, size 100 nm
Fm>2000, soluble in water,nonionic,size 10 nm
Fm<2000, soluble in in ethanol
Fm<2000 , soluble in water, ion-paired
Fm<2000, soluble in water, ionic
Choices:
ion -exchange
Bonded-reverse phase
Adsorption
Bonded normal phase
Molecular exculsion

Liquid chromatography is a separation technique that uses liquid stationary and mobile phases to separate and purify compounds. There are many different types of liquid chromatography, each with different stationary phases and separation mechanisms.

In ion-exchange chromatography, the stationary phase consists of ionizable groups that interact with ions in the mobile phase. This type of chromatography is used to separate charged molecules such as ions and ion-pair compounds.

In adsorption chromatography, the stationary phase consists of solid adsorbents like Silica or alumina that interacts with the analyte via van der Waals forces, dipole-dipole interactions, or hydrogen bonding. This type of chromatography is used to separate compounds based on their affinity for the stationary phase.

In normal-phase chromatography, the stationary phase is a non-polar material such as silica or alumina, and the mobile phase is a polar solvent such as water or methanol. This type of chromatography is used to separate non-polar compounds based on their affinity for the stationary phase.

In reversed-phase chromatography, the stationary phase is a polar material such as octadecyl silica or cyanide-bonded silica, and the mobile phase is a non-polar solvent such as hexane or methylene chloride. This type of chromatography is used to separate polar compounds based on their affinity with the stationary phase.

Molecular exclusion chromatography, also called size exclusion chromatography, is a type of chromatography that separates molecules based on their size. The stationary phase consists of porous spheres with different pore sizes, and molecules are separated based on their ability to penetrate the pores. This type of chromatography is used to separate molecules with molecular weights greater than 2,000 Daltons.