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The difference between the naturally occurring CRISPR system and the engineered CRISPR system used in the lab is there is a single guide RNA that interacts with Cas9 in the naturally occurring system.
The vital first step in genome editing is to precisely target a particular DNA sequence. Target sequences in biological systems can be found thanks to the interaction between the Cas9 protein and the guide RNA.
The Cas9 protein finds and cleaves the target DNA in CRISPR/Cas systems that are both natural and artificial. The six domains that make up the Cas9 protein are the REC I, REC II, Bridge Helix, PAM Interacting, HNH, and RuvC domains.
The interaction with guide RNA is carried out by Rec I, the biggest domain. The function of the REC II domain is currently unknown. The target DNA must start the cleavage process once it has attached to the arginine-rich bridging helix.
What is one difference between the naturally occurring CRISPR system and the engineered CRISPR system used in the lab?
a. Cas9 makes single-stranded cuts in the DNA in the naturally occurring system
b. there is a single guide RNA that interacts with Cas9 in the engineered system
c. the PAM is required only for the function of the engineered system
d. there is a single guide RNA that interacts with Cas9 in the naturally occurring system
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