When an action potential reaches the neuromuscular junction, acetylcholine is released at that synapse. Acetylcholine binds to nicotinic receptors concentrated in motor endplates, specialized regions of the post-synaptic membrane of muscle fibers.
Motor neurons connect to muscles at the neuromuscular junction where the synaptic terminals form a synaptic cleft with the motor endplate. The neurotransmitter acetylcholine diffuses across the synaptic cleft, causing sarcolemmal depolarization.
The neuromuscular junction is where the terminal portion of a motor neuron axon meets a sarcolemma separated by a synaptic cleft. Action potentials reaching the axon terminals trigger the release of acetylcholine leading to motor endplate depolarization.
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