Which of the following would be true if the water bath temperature were set higher than usual during a slot blot DNA quantitation?
A. Background signal would be increased.
B. DNA bands would be enhanced.
C. DNA bands would be enhanced and background signal would be reduced.
D. DNA bands would not be observed

Question

06-03-2024
Biology

contestada

Which of the following would be true if the water bath temperature were set higher than usual during a slot blot DNA quantitation?
A. Background signal would be increased.
B. DNA bands would be enhanced.
C. DNA bands would be enhanced and background signal would be reduced.
D. DNA bands would not be observed

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notyocheeze notyocheeze · Answer 1 · 2024-03-06T15:13:06+01:00

Answer: A. Background signal would be increased.

Explanation:

When the temperature is increased, it can lead to increased nonspecific binding of DNA probes to the membrane, which results in higher background signals. This is because higher temperatures can cause the DNA to become more flexible, allowing it to bind to unintended targets on the membrane more readily.

So, the correct option is A. Background signal would be increased.