Carbon fixation. A \text {CO}_2CO2C, O, start subscript, 2, end subscript molecule combines with a five-carbon acceptor molecule, ribulose-1,5-bisphosphate (RuBP). This step makes a six-carbon compound that splits into two molecules of a three-carbon compound, 3-phosphoglyceric acid (3-PGA). This reaction is catalyzed by the enzyme RuBP carboxylase/oxygenase, or rubisco.