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If the codon labeled x in the mrna strand shown below were changed to acg what effect would this have on the translation of the mrna stand

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base substitution mutation changes the DNA sequence and changes the mRNA during transcription;
the amino acid sequence is changed;
One codon is changed;
glutamic acid is changed to valine; 
shape of hemoglobin is changed;
less soluble and crystallizes out;
don't carry oxygen as well;
red blood cells sickle and impair the blood flow;
health problems such as anemia and fatigue

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