During glycolysis, glucose is converted to fructose through rearrangement of its atoms. Two phosphate molecules are then added to fructose at carbon number 1 and 6 to form fructose 1,6 biphosphate.
The phosphorylated fructose molecule then splits into 3-carbon molecules to form a couple of glucose -3- phosphate (G3P) molecules, each of which gains another phosphate molecule .
The two G3P molecules finally transfer their phosphate molecules to electron carriers and are reduced to form pyruvate. Thus glucose is converted to pyruvate.