Na has two strands. of the sequence of nucleotides of one strand was known, is it possible to use that information to determine the sequence of the second strand
Yes, because each of the four nucleotide bases present in DNA have a corresponding base: adenine bases always pair with thymine bases, and cytosine bases always pair with guanine bases. For example, if one strand of a DNA molecule was CTAGGT, the sequence of the second strand would be, from left to right, GATCCA.