Respuesta :

afathiah
1- Preparing the cDNA:
  a- Using two different tissue samples (such as control and cancer cells), isolate mRNA.
  b- For each sample, prepare complementry DNA (cDNA) from the isolated mRNA. Then fluorescent label cDNA of each sample with a different color and then mixed.

2- preparing the microarray:
  a- Bind DNA fragments of different genes to microarray wells.
  b- Attach ssDNA (single-stranded DNA) to microarray wells.

3- Combine the probe and the microarray samples:
Add labeled cDNA to the microarray wells. 

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Completing the flowchart attached below

1) Preparing the cDNA

  • Use two different sample tissues and isolate mRNA. example of sample tissues ; control and cancer cell.
  • Prepare cDNA from the isolated mRNA also label each cDNA for each with a different color and then mix.

2) Preparing the microarray :

  • Firstly bind the DNA fragments of the different genes to microarray wells
  • Attach the ssDNA to the microarray wells

3) Combine the probe and the microarray samples:

  • Addition of a labeled cDNA to microarray wells

Flowchart

Flow chart is the graphical representation of the processes undertaken in a sequential order to achieve a product or service. it is usually employed to simplify complex processes and to give a vivid explanation. The steps required to analyze gene activity using a microarray are as listed in the flowchart above.

Hence we can conclude that the completed flowchart showing the step required to analyze gene activity using a microarray is as listed above.

Learn more about microarray : https://brainly.com/question/25505636

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