Answer:
one is performing the freeze-thaw cycle to lyse the bacteria and pelleted the debris using a centrifuge then noticed that the pellet was fluoresce green
there can be some possibilities, such as-
- the GFP must have been covered up by the debris, that must have made the pellet fluoresce.
- there must be some non-lysed cells in the pellet as the thawing must have not been done efficiently.
- this inefficient freeze-thawing can make GFP to be pelleted down with cell debris rather than in the supernatant.
- because of all these E.coli would still contain some GFP protein.
