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The correct sequence for western blotting is:

1. Proteins are denatured by sodium dodecyl sulfate (SDS), and by boiling.
2.Transfer proteins to the nitrocellulose membrane.
3. Performing the polyacrylamide gel electrophoresis (PAGE).
4. Incubate the nitrocellulose membrane to primary antibodies and secondary antibodies subsequently.

a. 1,2,3,4
b. 4,1,3,2
c. 1,3,2,4
d. 1,3,2,4

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Answer:

3.page is this that answer

Explanation:

a.1,2,3,4

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The correct sequence for western blotting is: C. 1,3,2,4

Blotting can be defined as an analytical technique that is designed for the analysis of biological macromolecules such as:

  • Lipids.
  • Nucleic acid.
  • Proteins.
  • Carbohydrates.

For western blotting, the correct sequence of analyzing biological macromolecules such as proteins is:

1. The denaturation of protein through the use of sodium dodecyl sulfate (SDS) and by boiling.

2. The performance of polyacrylamide gel electrophoresis (PAGE).

2.The transfer of proteins to the nitrocellulose membrane.

4. Lastly, the incubation of the nitrocellulose membrane to subsequently form primary antibodies and secondary antibodies.

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