False. The electrophoresis buffer is NOT poured over the agarose gel because it DOES NOT charges the DNA samples so they can travel more readily across the chamber.
Gels used for DNA separation are frequently made of agarose, a polysaccharide that comes in the form of dry, powdered flakes. When agarose is subjected to heat in a buffer (salty water) and allowed to cool, it forms a solid, mildly squishy gel.
The DNA is filled into pre-cast wells in the gel and a current is generated to separate it using agarose gel electrophoresis. Because the phosphate backbone of the DNA (and RNA) molecule is negatively charged, DNA fragments will migrate to the positively charged anode when placed in an electric field.
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