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describe the construction of a recombinant plasmid containing the gene for luciferase and the insertion of the plasmid into a bacterial cell by placing the steps in order.
a. Perform a restriction digestion of the luciferase gene and the plasmid. b. Transform bacteria with the recombinant plasmid using electroporation. c. Ligate together the luciferase gene and the plasmid to generate a recombinant plasmid. d. Use PCR to amplify the gene for luciferase. e. Plate the bacterial cells, and screen for positive transformants.
1. First step 2. Last step

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The transformation procedure can be used to insert this plasmid into a bacteria.

Recombinant plasmids are injected into bacteria in what ways?

  • A plasmid vector can be modified by researchers to incorporate DNA snippets or genes, resulting in a recombinant plasmid.The transformation procedure can be used to insert this plasmid into a bacteria.
  • Bacteria can thus be employed as factories to copy vast amounts of DNA fragments since they reproduce quickly.Basic actions include:Cut the plasmid open, then "paste" the gene inside.DNA ligase and restriction enzymes, which cleave DNA, are both necessary for this process (which joins DNA).
  • Put the plasmid inside of bacterium.Plasmid-carrying bacteria should be multiplied and used as "factories" to produce the protein.

To learn more about  PCR  refer

https://brainly.com/question/7177771

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