The Rf value of a compound is generally equal to the distance that is traveled by the compound divided by the distance traveled by the solvent front.
Thin layer chromatography (TLC) is a widely used separation technique for the quantitative and qualitative analysis. It uses a thin layer of a stationary phase that is coated on a glass, plastic, or also on aluminum plate. A liquid solvent that is called the mobile phase generally carries the sample and separates it as it moves across the plate. It offers the advantages of simplicity, sensitivity, and rapid analysis over other separation techniques.
The stronger a compound is bound to the adsorbent , the slower it moves on the TLC plate. Non-polar compounds move up the plate most rapidly because of the higher Rf value, whereas polar substances will travel up the TLC plate slowly or not at all due to the lower Rf value.
The Rf value is a ratio, and it represents the relative distance between the spot traveled compared to the distance it could have traveled if it was moved with the solvent front. An Rf of 0.55 means the spot moved 55% as far as the solvent front, or a little more than halfway.
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