The right answer is A. it generates large amounts...
PCR is the acronym for Polymerase Chain Reaction, this Molecular Biology method was developed in 1985 by Kary Mullis, who obtained the Nobel Prize in Chemistry for this work in 1993. This revolutionary process coupled with the use of a heat-resistant DNA polymerase makes it possible to obtain, without cloning. , a considerable amplification of a given fragment of DNA.
The PCR reaction makes it possible to amplify in vitro a specific region of a given nucleic acid in order to obtain an amount sufficient to detect and study it.
To do this, a series of reactions allowing the replication of a double-stranded DNA template is repeated in a loop. Thus, during the PCR reaction, the products obtained at the end of each cycle serve as a template for the next cycle, the amplification is therefore exponential.
To have double-stranded DNA replication, you have to act in three steps.
* DNA must be denatured to obtain single-stranded matrices: denaturation.
* Borne and initiate replication of the sequence to be amplified using oligonucleotide specific primers: hybridization.
* Perform the polymerization reaction of the complementary strand. At the end of each cycle, the products are in the form of double-stranded DNA: polymerization.
The three steps, constituting a PCR cycle, are carried out at different temperatures to control the enzymatic activity.
