You are using a spectrophotometer to measure the level of fucoxanthin (a pigment imparting the distinctive green-brown color in brown algae) in Giant Kelp blades versus bladders. The kelp blade or bladder was washed, and chopped up into a small amount of water. It was then disrupted with the addition of a mixture of detergent and acetone. The mixture was filtered to remove debris and the resulting extraction is measured in the spectophotometer.
Select all components needed for an appropriate blank.

1) Salt
2) Minimal Media
3) Detergent
4) Salt Water
5) Acetone
6) Fucoxanthin
7) dH2O
8) kelp bladder